The scientific technique and practice of measuring fluorescence to quantify or identify chemical and biological substances in a sample.
From fluoro- + Greek metria, 'measurement'. This term solidified in the 1950s-60s as fluorescence became a standard analytical method in chemistry and biology.
Fluorometry transformed biochemistry by allowing researchers to measure things that used to be invisible—hormones, antibodies, pollutants—all by watching them glow under controlled light.
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